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Ultrafast synthesis of gold nanosphere cluster coated by graphene quantum dot for active targeting PA/CT imaging and near-infrared laser/pH-triggered chemo-photothermal synergistic tumor therapy
摘要： In this work, simple raw materials and reaction conditions were used to synthesize a versatile nanoprobe using a one-step method. Graphene quantum dot (GQD) and gold chloride were mixed and irradiated with ultra-violet (UV) radiation for 1 min. Then, the gold nanosphere cluster with the diameter of 50 nm and coated using GQD was formed using a facile one-step approach. GQD played the roles of reducing agent, stabilizer and drug carrier instead of a harmful reducing agent or stabilizer. The nanoprobe had good dispersion, stability, excellent photoacoustic imaging (PAI) and computed tomography (CT) imaging performance, low cytotoxicity and photothermal conversion e?ciency of up to 51.31%. The results for cell and animal experiments showed that targeted PAI/CT imaging of tumor after modi?cation of folic acid (FA) could be obtained using the probe. Meanwhile, after the adsorption of doxorubicin, the chemo-photothermal combined therapy for tumor could be carried out through controlled drug release from GQD under heated and acidic environment of tumor. Additionally, the treatment e?ect was signi?cantly superior to single modes. The body weight, Hematoxylin and Eeosin (H&E) staining of main organs and blood biochemical indicators showed that the probe had good biological safety after injection. The current work proposes a new dual-mode bio-imaging and chemo-photothermal combined therapy nanoprobe, which presents good application prospect for tumor theragnostic.
关键词： CT imaging,One-step synthesis,Drug release,Photoacoustic imaging,Chemo-photothermal therapy
更新于2025-11-25 10:30:42
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Polydopamine-functionalized black phosphorus quantum dots for cancer theranostics
摘要： Black phosphorus (BP) is a promising theranostic agent owing to its excellent photothermal property, biocompatibility and biodegradability. However, the rapid degradation of BP with oxygen and moisture causes the innate instability that is the Achilles’ heel of BP, hindering its further applications in cancer theranostics. Herein, a facile surface passivation strategy was developed to prepare polydopamine (PDA) coated BP quantum dots (QDs) (denoted as BP@PDA) through self-polymerization method. PDA with enriched phenol groups plays as a scavenger of reactive oxygen, which can efficiently prevent the oxidation of BP quantum dots and make them much stable in water (～90% for BP@PDA vs. only 10% for pure BP QDs after 10 days storage). Furthermore, PDA with strong near-infrared (NIR) absorption could greatly improve the photothermal conversion efficiency (PCE) of BP QDs from 22.6% to 64.2% (～2.84-fold higher). Considering the excellent biodegradability and good biocompability of both BP QDs and PDA, the as-prepared BP@PDA hold great potential for cancer theranostics.
关键词： Photoacoustic imaging,Black phosphorus,Photothermal therapy,Cancer theranostics,Polydopamine
更新于2025-11-19 16:56:42
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<p>A multifunctional-targeted nanoagent for dual-mode image-guided therapeutic effects on ovarian cancer cells</p>
摘要： Nanomedicine has emerged as a novel therapeutic modality for cancer treatment and diagnosis. Lipid–polymer hybrid nanoparticles (LPHNPs) are core–shell nanoparticle (NP) structures comprising polymer cores and lipid shells, which exhibit complementary characteristics of both polymeric NPs and liposomes. However, it is difficult to wrap perfluoropentane (PFP) into core–shell NPs in the existing preparation process, which limits its application in the integration of diagnosis and treatment. Methods: The folate-targeted LPHNPs-loaded indocyanine green/PFP-carrying oxygen (TOI_HNPs) using a combination of two-step method and solution evaporation technique for the first time. The essential properties and dual-mode imaging characteristics of developed NPs were determined. The cellular uptake of TOI_HNPs was detected by confocal microscopy and flow cytometry. The SKOV3 cell viability and apoptosis rate were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry. The ROS was demonstrated by fluorescence microplate reader and the expression of hypoxia-inducible factor 1-alpha (HIF-1α) and IL-6 was detected by Western blot. Results: TOI_HNPs showed spherical morphology with particle size about (166.83±5.54) nm and zeta potential at -(30.57±1.36) mV. It exhibited better stability than lipid NPs and higher encapsulation efficiency as well as active targeting ability than poly (lactic-co-glycolic acid) (PLGA) NPs. In addition, the novel NPs could also act as the contrast agents for ultrasound and photoacoustic imaging, providing precision guidance and monitoring. Furthermore, TOI_HNPs-mediated photo–sonodynamic therapy (PSDT) caused more serious cell damage and more obvious cell apoptosis, compared with other groups. The PSDT mediated by TOI_HNPs induced generation of intracellular ROS and downregulated the expression of HIF-1α and IL-6 in SKOV3 cells. Conclusion: This kind of multifunctional-targeted nanoagent may provide an ideal strategy for combination of high therapeutic efficacy and dual-mode imaging guidance.
关键词： core-shell nanoparticle,ultrasound,photo-sonodynamic therapy,phase transformation,photoacoustic imaging,laser
更新于2025-11-14 17:03:37
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Photoacoustic temperature imaging based on multi-wavelength excitation
摘要： Building further upon the high spatial resolution offered by ultrasonic imaging and the high optical contrast yielded by laser excitation of photoacoustic imaging, and exploiting the temperature dependence of photoacoustic signal amplitudes, this paper addresses the question whether the rich information given by multispectral optoacoustic tomography (MSOT) allows to obtain 3D temperature images. Numerical simulations and experimental results are reported on agarose phantoms containing gold nanoparticles and the effects of shadowing, reconstruction flaws, etc. on the accuracy are determined.
关键词： photoacoustic imaging,absorption coefficient,finite difference,multispectral optoacoustic tomography
更新于2025-11-14 15:30:11
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Spectral analysis for photoacoustic pressure sensor designs: Theoretical model improvement and experimental validation
摘要： In the pulsed laser photoacoustic (PA) detection and spectroscopy applications, the fundamental frequency of the PA signal produced, and the sensor resonance frequency should be as close as possible to each other so that analyzes from the obtained signals can be performed effectively. In order to determine the fundamental frequency of the PA wave, a theoretical model approach based on the development of the frequency domain solution of the PA wave equation is presented for use in the PA pressure sensor designs. For the validation of the theoretical model approach, a PA experimental setup was established, and measurements were made in distilled water. The theoretical and experimental PA frequency spectra were determined to be very compatible with each other. Thus, the theoretical model approach was experimentally validated. According to the theoretical model approach, fundamental frequency values obtained from the experimental measurement results were determined with an average accuracy of ? 4.212%. Furthermore, it has been determined that this value has fallen to ?0.267% in the measurements. With the obtained results from the theoretical model approach, we propose that PA pressure sensors with the more selective and narrower band can be designed for more sensitive detection. Moreover, in this study the effects of different laser parameters such that pulse duration, and laser beam width, on the spectral content of the obtained PA signal are analyzed. These analyses will shed light on the vision of acoustic pressure sensor design by helping to select the most optimum parameters for PA detection.
关键词： Theoretical model approach,Photoacoustic wave equation,Acoustic pressure sensor,Frequency domain solution,Pulsed laser photoacoustic method,Spectroscopy
更新于2025-09-23 15:23:52
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Enhancement of objects in photoacoustic tomography using selective filtering
摘要： Here we developed a real-time photoacoustic tomography (PAT) imaging acquisition device based on the linear array transducer utilized on ultrasonic devices. Also, we produced a phantom including diverse contrast media and acquired PAT imaging as the light source wavelength was changing to see if the contrast media reacted. Indocyanine green showed the highest reaction around the 800-nm band, methylene blue demonstrated the same in the 750-nm band, and gold nanoparticle showed the same in the 700-nm band. However, in the case of superparamagnetic iron oxide, we observed not reaction within the wavelength bands used herein to obtain imaging. Moreover, we applied selective filtering to the acquired PAT imaging to remove noise from around and reinforce the object’s area. Consequentially, we could see the object area in the imaging was effectively detected and the image noise was removed.
关键词： Photoacoustic image,real-time,back projection algorithm,selective filtering,ultrasonic imaging
更新于2025-09-23 15:23:52
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Fiber Optic Sensors Based on Photoacoustic Effect for Rebar Corrosion Measurement
摘要： An all-optical ultrasound sensing system based on photoacoustic principle is developed to monitor and investigate the initiation of early stage steel rebar corrosion in real time. The ultrasound generator was developed by small size gold nanocomposites coated optical fibers to generate 8-MHz bandwidth signals. The ultrasound receiver was applied by a fiber Bragg grating sensor. This system was employed for the first time as a disruptive approach in order to replace the application of conventional electrical-based sensors due to their unique advantages including miniature size, lightweight, immunity to electromagnetic interference, resistance to corrosion, and distributed sensing capability. Accelerated corrosion tests were conducted on steel rebars to validate the developed sensing system. The obtained results indicated that the mass loss of corroded steel rebars was characterized by the centroid frequency shift of measured ultrasound signals. The correlation between the centroid frequency shift and the mass loss depicted an exponential relationship.
关键词： photoacoustic (PA) principle,fiber optic sensor (FOS),frequency shift,Corrosion monitoring
更新于2025-09-23 15:22:29
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Ordered assemblies of Fe3O4 and a donor-acceptor-type π-conjugated polymer in nanoparticles for enhanced photoacoustic and magnetic effects
摘要： We report that the ordered structure in the assemblies of iron oxide nanoparticles in conjugated polymer nanoparticles is the key to achieve better properties to realize multimodal theranostic agents for magnetic resonance and photoacoustic imaging. Hybrid nanoparticles of a conjugated polymer (PCPDTBT), a phospholipid (D8PE) with a primary amine polar head, and iron oxide (Fe3O4) nanoparticles were prepared by a phase-separated film shattering process by varying the iron oxide concentration while maintaining a fixed mixing ratio of PCPDTBT and D8PE. Notably, the hybrid nanoparticles assembled at a molar mixing ratio of 1:1:0.8 (PCPDTBT/D8PE/Fe3O4) exhibited the shortest transversal relaxation time, T2, and a photoacoustic signal 22 times higher than that obtained at the 1:1:0 mixing ratio. Structural analysis by X-ray diffraction together with the measurements of energy transfer by transient absorption spectroscopy confirmed that the structural ordering of these hybrid nanoparticles was responsible for their enhanced photoacoustic and magnetic properties.
关键词： Conjugated polymers,Photoacoustic imaging,Magnetic resonance imaging,Iron oxides,Hybrid nanoparticles
更新于2025-09-23 15:22:29
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In Vivo 3D Imaging of Retinal Neovascularization Using Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Imaging
摘要： The pathological process of neovascularization of the retina plays a critical role in causing vision loss in several diseases, including diabetes, retinal vein occlusion, and sickle cell disease. Retinal neovascularization can lead to vitreous hemorrhage and retinal detachment, yet the pathological process of neovascularization is a complex phenomenon under active investigation. Understanding and monitoring retinal neovascularization is critically important in clinical ophthalmology. This study describes a novel multimodal ocular imaging system which combines photoacoustic microscopy (PAM) and a spectral domain optical coherence tomography (SD-OCT) to improve the visualization of retinal neovascularization (RNV), their depth, and the surrounding anatomy in living rabbits. RNV was induced in New Zealand rabbits by intravitreal injection of vascular endothelial growth factor (VEGF). The retinal vasculature before and after injection at various times was monitored and evaluated using multimodal imaging including color fundus photography, fluorescein angiography (FA), OCT, and PAM. In vivo experiments demonstrate that PAM imaging distinctly characterized the location as well as the morphology of individual RNV with high contrast at a safe laser energy of 80 nJ. SD-OCT was used to identify a cross-sectional structure of RNV. In addition, dynamic changes in the retinal morphology and retinal neovascularization were observed at day 4, 5, 6, 7, 9, 11, 14, 28, and day 35 after VEGF injection. PAM demonstrated high-resolution optical absorption of hemoglobin and vascular imaging of the retina and choroid with increased depth of penetration. With the current multimodal imaging system, RNV can be easily visualized in both 2D and 3D angiography. This multimodal ocular imaging system provides improved characterization of the microvasculature in a safe manner in larger rabbit eyes.
关键词： PAM,VEGF,multimodal imaging,optical coherence tomography,photoacoustic microscopy,retinal neovascularization,vascular endothelial growth factor,OCT
更新于2025-09-23 15:22:29
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Fish oil preparation inhibits leukocyte recruitment and bands that characterize inflamed tissue in a model of phenol-induced skin inflammation: percutaneous penetration of a topically applied preparation demonstrated by photoacoustic spectroscopy
摘要： Fish oil (FO) is a natural source of omega-3 fatty acids, with well-established beneficial effects in inflammatory diseases when FO is orally administered. This study investigated the effects of a topically applied FO preparation (FOP) on phenol-induced ear edema and evaluated the percutaneous penetration of FOP in ear tissue. After applying phenol, groups of mice received FOP on the ear. After 1 h, ear tissue was collected to determine the percent inhibition of edema, myeloperoxidase activity, and to perform photoacoustic spectroscopy (PAS). Treatment with FOP did not reduce edema, but reduced myeloperoxidase activity. The FOP decreased the area of bands that characterize inflamed tissue and penetrated into the tissue. These results indicated an inhibitory effect of FOP on leukocyte recruitment in phenol-induced ear edema. These data support the applicability of PAS as a non-destructive method for evaluating the inflammatory response, percutaneous penetration and antiinflammatory activity of compounds.
关键词： ear edema,Fish oil,inflammation,phenol,skin,photoacoustic spectroscopy
更新于2025-09-23 15:22:29