- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Y-shaped DNA-Mediated hybrid nanoflowers as efficient gene carriers for fluorescence imaging of tumor-related mRNA in living cells
摘要： Organic-inorganic hybrid nanomaterial has gained much attention due to its excellent performances in bioanalysis and biomedicine. However, the preparation of DNA-inorganic hybrid nanomaterial with suitable size for cell uptake remains a huge challenge. Herein, a moderate biomineralization strategy for synthesis of Y-DNA@Cu3(PO4)2 (Y-DNA@CuP) hybrid nanoflowers is reported. Y-DNA with a loop structure is used as both the biomineralization template and the recognition unit for thymidine kinase 1 (TK1) mRNA. The Y-DNA probe can linearly response to TK1 mRNA target sequence in a range from 2 nM to 150 nM with the limit of detection as low as 0.56 nM. Interestingly, the presence of Y-DNA significantly decreases the size of Cu3(PO4)2 (CuP) particles, which allows them suitable for intracellular applications as gene nanocarriers. Once inside the cells, the hybrid nanoflowers dissolve and release the Y-DNA probes. Then, the intracellular TK1 mRNA hybridizes with the loop region of Y-DNA, which dissociates the Cy3-labeled loop strand and turns on the red fluorescence. Through the real-time imaging of the intracellular TK1 mRNA, the assessment of tumor cells before and after the treatment of drugs including b-estradiol and tamoxifen is achieved.
关键词： Cell imaging,Thymidine kinase 1,Biomineralization,Y-shaped DNA@CuP,Tumor-related biomarker
更新于2025-09-19 17:15:36
-
Sulfur-rich carbon dots as a novel fluorescent imaging probe for distinguishing the pathological changes of mouse-bone cells
摘要： Nowadays, the fluorescent carbon dots (C-dots), as a type of heavy-metal-free nanomaterials, have been widely suggested for biological imaging, but attempts made for detecting and distinguishing bone-related diseases are still rarely reported by previous works. Here, a type of C-dots, featuring rich sulfur content and excellent bone cell affinity, is prepared by one-pot hydrothermal method. Various characterization techniques have been employed to study the structure and fluorescent properties of the sulfur C-dots, which include the X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR), ultraviolet-visible (UV–vis) absorption, and fluorescence spectra. Our results show that the sulfur C-dots are featured with small particle size at ~4 nm and rich sulfur content as high as 24.85%, allowing the C-dots to exhibit excellent stability in the aqueous solution and good compatibility and affinity with bone cells. Moreover, we find the sulfur rich C-dots as the excitation wavelength changes from 270 nm to 460 nm can exhibit emission-tunable colors from blue, green and to yellow, showing a rich choice of fluorescent colors for cell imaging. Together with the advantages of low cytotoxicity behavior and sulfur-rich functionalized groups, the sulfur C-dots have been proposed to act as a fluorescent probe for detecting and distinguishing bone-related diseased cells where bone-related normal and diseased cells after incubated with sulfur rich C-dots are found to emit blue and yellow fluorescence upon excitation at 405 nm and 488 nm, respectively. Unlike previous works that are always reported to use the C-dots for cell imaging, here we have presented new application possibilities of C-dots for distinguishing the diseased cells from the normal cells.
关键词： Cell imaging,Sulfur,Tunable fluorescence,C-dots,One pot method
更新于2025-09-19 17:15:36
-
Facile Fabrication of Fluorescent Inorganic Nanoparticles with Diverse Shapes for Cell Imaging
摘要： In the present work, we describe a facile and general method of fabricating fluorescent inorganic nanoparticles with diverse shapes for cell imaging application. The hematite (α-Fe2O3) nanoparticles (HNPs) with three different shapes (i.e., spindle shape, ellipsoidal shape and quasi-spherical shape) were first prepared as model systems in consideration of good biocompatibility and the controllable morphology of α-Fe2O3. Three fluorescent HNPs with different shapes were achieved via one-pot sol-gel reaction of AIEgen-functionalized siloxane (AIEgen-Si(OCH3)3) and TEOS in the presence of PVP-stabilized HNPs. Due to the fluorescence originating from the thin AIEgens-contained SiO2 shell around the HNPs, their photoluminescent intensities can be tuned by changing the concentrations of TEOS and AIEgen-Si(OCH3)3 in feed prior to the sol-gel reaction. When the as-prepared fluorescent products were dispersed in water, they gave intense green light emission upon excitation at 360 nm with relatively high fluorescence quantum yield. Further, fluorescent HNPs exhibited low cytotoxicity and excellent photostability and, thus, were used as optical probes to preliminarily explore the effect of nanoparticle shapes on their cellular uptake behaviors. This work should open a facile way to prepare various fluorescent inorganic nanoparticles with specific morphology for various biological applications.
关键词： inorganic nanoparticles,fluorescent probes,cell imaging,shape
更新于2025-09-19 17:15:36
-
Efficient “Ratiometric” fluorescence chemosensor for selective detection of Hg2+ ions based on phosphonates: its live cell imaging and molecular keypad lock applications
摘要： We report that the smart chemosensors (L1 and L2) can detect Hg2+ ion selectively over other metal ions was studied by various spectral techniques. Particularly, the proposed chemosensors exhibiting the notable color and spectra changes with the addition of mercury ions. Moreover, the colorimetric and fluorescence could be reversed with the addition of EDTA into the L1-Hg2+ and L2-Hg2+ solutions. Based on the admirable reversibility process, we displayed a molecular-scale sequential information processing circuit proposed the “Writing-Reading-Erasing-Reading” and “Multi-write” behaviors in the form of binary logic. Furthermore, L1 and L2 mimic as a molecular keypad lock and molecular logic gates with the successive addition of Hg2+ and EDTA. The binding ability of Hg2+ ion with L1 and L2 was further demonstrated by Density Functional Theory (DFT) studies. The biocompatibility of these chemosensors is successfully used for live cell imaging of Hg2+ in HeLa cells under the experimental conditions. Furthermore, the sensors L1 and L2 were also useful to determine the concentration of Hg2+ ion in real water samples to exhibit its practical applications.
关键词： Cell imaging,Molecular keypad lock,Chemosensors,Turn-off Fluorescence,Mercury (II),Logic gates
更新于2025-09-19 17:15:36
-
Fluorescence sensing and intracellular imaging of Pd2+ ion by a novel coumarinyl-rhodamine Schiff base
摘要： Coumarinyl-rhodamine, HCR, serves as extremely selective sensor to Pd2+ ion in ethanol/H2O (8:2, v/v, HEPES buffer, pH 7.2) solution and the limit of detection (LOD) is 18.8 nM (3σ method). The free sensor, HCR, is weakly emissive and in presence of Pd2+, the colour changes from straw to pink with very strong emission at 598 nm in presence of eighteen other cations. The plausible mechanism includes opening of the spirolactam ring of rhodamine upon interaction with Pd2+. This is justified by a structure optimization and transition energy calculation by DFT technique. HCR undergoes 1:1 complexation with Pd2+ that has been confirmed via Job’s plot, mass spectra and Bensei-Hildebrand plot (association constant Ka, 9.1×10^4 M^-1). A separate in vitro experiment shows that HCR can specifically perceive Pd2+ in MCF7 (Human breast adenocarcinoma) cell lines.
关键词： Coumarinyl-rhodamine,spirolactam ring opening,DFT computation,Pd2+-sensor,live cell imaging
更新于2025-09-19 17:15:36
-
Rhodamine-assisted fluorescent strategy for the sensitive and selective in-field mapping of environmental pollutant Hg(II) with potential bioimaging
摘要： Mercury is a global pollutant that can damage human brain, central nervous system and endocrine moreover, other biological systems. Its real-time monitoring is a key challenge in the control of mercury emissions or pollution. In this study, a simple and efficient fluorescent sensor (PST) comprising of rhodamine B and 2-amino-5-bromopyridine was developed for the detection of environmental toxicant Hg2+. The newly synthesized sensor displayed a highly selective and sensitive 'turn-on' response by the addition of Hg2+ among miscellaneous metal cations (Ag+, Al3+, Ba2+, Ca2+, Cd2+, Fe3+, Fe2+, K+, Li+, Mg2+, Mn2+, Cu2+, Pb2+, Ni2+, Na+ and Zn2+) in CH3CN: H2O (8:2 v/v) system. The 1:1 binding stoichiometry, limit of detection (LOD) 0.63 μM along with binding constant of 1.04 × 105 M?1 was calculated for the complex PST-Hg2+. The reversible nature of the complex was confirmed in the presence of ethylenediaminetetraacetate (EDTA). The significant enhancement of fluorometric and absorption response was attributed to the spirolactam ring opening via photo-induced electron transfer (PET) of xanthene moiety. This spirolactam ring opening was further verified by density functional theory, 1H NMR and Fourier transform infrared spectroscopy (FT-IR). The real-time applicability of the sensor in environmental and biological fields was proved as detection kit on TLC strips and with the help of Laser confocal microscopy for live cell imaging by using HeLa cells. Conclusively, PST can be regarded as accurate 'two in one' kit for colorimetric recognition of Hg2+ in the environment and fluorescent cell imaging in live HeLa cells.
关键词： Rhodamine,High sensitivity,Colorimetric,Cell imaging,Infield,Toxic metal
更新于2025-09-19 17:15:36
-
An efficient new dual fluorescent pyrene based chemosensor for the detection of bismuth (III) and aluminium (III) ions and its applications in bio-imaging
摘要： A new simple pyrene based schiff base chemosensor 1 (nicotinic acid pyren-1-ylmethylene-hydrazide) has been constructed and is prepared from 1-pyrenecarboxaldehyde and nicotinic hydrazide. Notably, the chemosensor 1 exhibited remarkable colour changes while in the presence of trivalent metal ions like Bi3+ & Al3+ ion in DMSO-H2O, (1:1 v/v, HEPES = 50 mM, pH = 7.4). The UV-Vis spectral investigation of chemosensor 1 showed that the maximum absorption peak appeared at 378 nm. In emission studies, chemosensor 1 develops weak fluorescence, while upon the addition of Bi3+ and Al3+ ions, it exhibits an enhancement of fluorescence intensity. Nevertheless, rest of metal ions have no changes in the emission spectra. The association constant of chemosensor 1 for binding to Bi3+ & Al3+ system had a value of 1.27 × 104 M-1 and 1.53 × 104 M-1. The detection limits were 0.12 μM for Bi3+ and 0.17 μM for Al3+ respectively. The overall results reveal that chemosensor 1 can act as a dual-channel, highly selective, and sensitive probe for Bi3+ and Al3+ ions. Moreover, the fluorescence imaging of chemosensor 1 was applied in RAW 264.7 cell line and cytotoxicity assay prove that this chemosensor 1 is non-toxic as well as highly biocompatible.
关键词： Pyrene Schiff base,Spectroscopic studies,Live cell imaging,PET,Bi3+ and Al3+ detection
更新于2025-09-19 17:15:36
-
Enzymatic Preparation of Plasmonic-Fluorescent Quantum Dot-Gold Hybrid Nanoprobes for Sensitive Detection of Glucose and Alkaline Phosphatase and Dual-Modality Cell Imaging
摘要： Herein, we develop a route to prepare bifunctional plasmonic-fluorescent quantum dot-gold (QD-Au) hybrid nanoprobes by use of enzymatic reactions. Two bio-enzymes, glucose oxidase (GOx) and alkaline phosphatase (ALP) were chosen for the enzymatic preparation of core-satellite or core-shell type CdSe/ZnS@Au hybrid nanostructures. The enzymatic products, H2O2 and L-Ascorbic acid, of the two enzymes were exploited as mild reducing agents for controlled Au deposition on QD surfaces. The polymer multilayers by layer-by-layer assembly were used to adjust the separation between QD core and plasmonic Au, which can effectively reduce the quenching effect of the Au on QDs. The as-prepared QD@Au hybrid nanostructures are excellent dual-modality imaging nanoprobes, and can be used for fluorescence and dark-field scattering dual-imaging of MCF-7 cells. More importantly, the two enzymatic reaction systems can be explored for sensitive and selective detection of glucose and alkaline phosphatase, respectively, by monitoring the fluorescence spectra change of QD@Au hybrid nanoparticles, which is very useful for the glucose- and ALP-related disease diagnosis.
关键词： Biosensing,Fluorescence,Cell imaging,Enzymatic preparation,Plasmonic
更新于2025-09-19 17:13:59
-
Systematic investigation of bioorthogonal cellular DNA metabolic labeling in a photo-controlled manner
摘要： Two 5-ethynyl-2′-deoxyuridine (EdU) derivatives photocaged on N-3 or 3′ position were synthesized by simple and effective methods, through direct UV irradiation on cells treated with them, cellular DNA metabolic labeling was successfully controlled. The systematic investigation of optical-cleavage position and type in living systems contributes to form a more integrated repertoire of bioorthogonal cleavage reaction.
关键词： DNA metabolic labeling,Cell imaging,Photocage group,Modified nucleoside,Bioorthogonal chemistry
更新于2025-09-19 17:13:59
-
Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes
摘要： A compact lensless microscope comprising a custom-made LED engine and a CMOS imaging sensor has been developed for live-cell culture imaging inside a cell incubator environment. The imaging technique is based on digital inline-holographic microscopy, while the image reconstruction is carried out by angular spectrum approach with a custom written software. The system was tested with various biological samples including immortalized mouse astrocyte cells inside a petri dish. Besides the imaging possibility, the capability of automated cell counting and tracking could be demonstrated. By using image sensors capable of video frame rate, time series of cell movement can be captured.
关键词： lensless holographic microscopy,cell imaging,LED,CMOS image sensor,cell culture,cell counting
更新于2025-09-16 10:30:52