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Ultrafast synthesis of gold nanosphere cluster coated by graphene quantum dot for active targeting PA/CT imaging and near-infrared laser/pH-triggered chemo-photothermal synergistic tumor therapy
摘要： In this work, simple raw materials and reaction conditions were used to synthesize a versatile nanoprobe using a one-step method. Graphene quantum dot (GQD) and gold chloride were mixed and irradiated with ultra-violet (UV) radiation for 1 min. Then, the gold nanosphere cluster with the diameter of 50 nm and coated using GQD was formed using a facile one-step approach. GQD played the roles of reducing agent, stabilizer and drug carrier instead of a harmful reducing agent or stabilizer. The nanoprobe had good dispersion, stability, excellent photoacoustic imaging (PAI) and computed tomography (CT) imaging performance, low cytotoxicity and photothermal conversion e?ciency of up to 51.31%. The results for cell and animal experiments showed that targeted PAI/CT imaging of tumor after modi?cation of folic acid (FA) could be obtained using the probe. Meanwhile, after the adsorption of doxorubicin, the chemo-photothermal combined therapy for tumor could be carried out through controlled drug release from GQD under heated and acidic environment of tumor. Additionally, the treatment e?ect was signi?cantly superior to single modes. The body weight, Hematoxylin and Eeosin (H&E) staining of main organs and blood biochemical indicators showed that the probe had good biological safety after injection. The current work proposes a new dual-mode bio-imaging and chemo-photothermal combined therapy nanoprobe, which presents good application prospect for tumor theragnostic.
关键词： CT imaging,One-step synthesis,Drug release,Photoacoustic imaging,Chemo-photothermal therapy
更新于2025-11-25 10:30:42
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Rapidly self-heating shape memory polyurethane nanocomposite with boron-doped single-walled carbon nanotubes using near-infrared laser
摘要： In this study, boron-doped single-walled carbon nanotubes (SWCNTs) were synthesized by high-temperature heat treatment (1300 °C) with a boric acid precursor and SWCNTs instead of the conventional chemical doping process. Then, these boron-doped single-walled carbon nanotubes (B-SWCNTs) were added to polyurethane to prepare polyurethane nanocomposites having excellent thermal and mechanical properties. Changes in properties that occurred due to structural changes inside the composite were investigated as the added amount of nanofiller was increased. In particular, a near-infrared (NIR) laser (808 nm) was directly irradiated on the nanocomposite film to induce photothermal properties on the surface of the B-SWCNTs. In the case of the PU nanocomposite film with a filler content of 3 wt%, a self-heating film material that rapidly heated to 250 °C within 10 s was developed. The newly developed material can be applied to electronic devices and products as a heat-generating coating material, de-icing of airplane, a heat sink, for bio-sensing, etc., using a moulding process.
关键词： boron-doping,photothermal,thermoelectrics,carbon nanotube,polyurethane
更新于2025-11-25 10:30:42
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Antiangiogenesis-Combined Photothermal Therapy in the Second Near-Infrared Window at Laser Powers Below the Skin Tolerance Threshold
摘要： Photothermal agents with strong light absorption in the second near-infrared (NIR-II) region (1000–1350 nm) are strongly desired for successful photothermal therapy (PTT). In this work, titania-coated Au nanobipyramids (NBP@TiO2) with a strong plasmon resonance in the NIR-II window were synthesized. The NBP@TiO2 nanostructures have a high photothermal conversion efficiency of (93.3 ± 5.2)% under 1064-nm laser irradiation. They are also capable for loading an anticancer drug combretastatin A-4 phosphate (CA4P). In vitro PTT studies reveal that 1064-nm laser irradiation can efficiently ablate human lung cancer A549 cells and enhance the anticancer effect of CA4P. Moreover, the CA4P-loaded NBP@TiO2 nanostructures combined with PTT induce a synergistic antiangiogenesis effect. In vivo studies show that such CA4P-loaded NBP@TiO2 nanostructures under mild 1064-nm laser irradiation at an optical power density of 0.4 W cm?2, which is lower than the skin tolerance threshold value, exhibit a superior antitumor effect. This work presents not only the development of the NBP@TiO2 nanostructures as a novel photothermal agent responsive in the NIR-II window but also a unique combined chemo-photothermal therapy strategy for cancer therapy.
关键词： Antiangiogenesis therapy,Gold nanobipyramids,Plasmon resonance,Core@shell nanostructures,Photothermal therapy
更新于2025-11-21 11:24:58
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Nanogold-core Multifunctional Dendrimer for Pulsatile Chemo-, Photothermal- and Photodynamic- Therapy of Rheumatoid Arthritis
摘要： This investigation reports a novel nanoGold-core multifunctional dendrimer for pulsatile chemo-, photothermal- and photodynamic- therapy of rheumatoid arthritis (RA). Architecturally, the nanocomposites comprised of a nanoGold (Au) at the focal whose surface is functionalized by hydroxy-terminated thiolated-dendrons following Au-thiol bond formation to produce nanoGold-core multifunctional dendrimer (Au-DEN). The surface hydroxyl groups of Au-DEN were then conjugated with methotrexate (MTX; a disease-modifying first line anti-rheumatic drug; DMARD; 74.29±0.48 % loading) to form Au-DEN-MTX-NPs (Particle size: 100.15±28.36 nm; poly dispersibility index, PDI: 0.39±0.02; surface zeta potential, ζ: -22.45±1.06 mV). MTX was strategically selected to serve as an anti-rheumatic DMARD as well as a targeting ligand to attain selective localization of the formulation in arthritic tissue via folate receptors upregulated on arthritic tissues. The docking study was performed to confirm the viable binding efficiency of MTX towards β-folate receptors that are overexpressed on arthritic tissues taking folic acid as a reference standard. The IR780, a NIR active bioactive was also loaded in Au-DEN-MTX NPs to offer photothermal benefit upon irradiation with NIR laser (wavelength: 808 nm). The hypothesis was tested by elucidation of in vitro drug release profile, photothermal activity, cellular uptake (Fluorescence and confocal laser scanning microscopy; CLSM), cell viability assay (MTT protocol) and Intracellular reactive oxygen species (ROS) generation in mouse macrophage RAW264.7 cells and Lipopolysaccharide (LPS) activated RAW264.7 cells. Furthermore, the hemolytic toxicity and stability studies were also investigated to determine the blood compatibility as well as ideal storage condition of NPs. The outcome of this investigations presents developed multifunctional targeted NPs to be potential therapeutics for the improved treatment of RA. The approach can also be applied to other clinical interventions involving countering inflammatory conditions.
关键词： Rheumatoid arthritis,NanoGold-core multifunctional dendrimer,Methotrexate,Photothermal,Near-infrared,Dendron
更新于2025-11-21 11:24:58
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Differential photothermal and photodynamic performance behaviors of gold nanorods, nanoshells and nanocages under identical energy conditions
摘要： Various gold (Au) nanostructures have shown promising near infrared (NIR) light-activated phototherapeutic effects; however, their reported photothermal or photodynamic performance behavior is usually inconsistent or even conflicted, dramatically limiting the improvement of phototherapeutic Au nanostructures. The potential reason for this uncertainty is mainly because the photoactivities of Au nanostructures are not evaluated under identical energy conditions. Herein, three Au nanostructures, Au nanorods (NRs), nanoshells (NSs), and nanocages (NCs), were prepared to provide the same localized surface plasmon resonance (LSPR) peaks at 808 nm. All these Au nanostructures (at the same optical density) could fully exert their photoactivities under the identical and optimal energy condition of 808 nm laser irradiation. It was found that these Au nanostructures could induce similar levels of temperature elevation but different levels of reactive oxygen species (ROS) production, where Au NCs exhibited the highest ROS production, followed by Au NSs and NRs. In vitro and in vivo phototherapeutic assessments further supported that Au NCs could cause the most severe cell death and tumor growth regression. This means that the identical incident energy has different contribution to photothermal and photodynamic performance of Au nanostructures, and the corner angle structures of Au NCs compared with NSs and NCs could more efficiently convert the photon energy into photodynamic property. Taken all together, Au NCs hold great potential for phototherapy due to their efficient energy utilization capability.
关键词： reactive oxygen species,photodynamic therapy,gold nanostructures,photothermal therapy,cancer treatment
更新于2025-11-21 11:08:12
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Hyaluronic acid functionalized nanoparticles loaded with IR780 and DOX for cancer chemo-photothermal therapy
摘要： IR780 is a near infrared (NIR) dye with a huge potential to be applied in cancer phototherapy and imaging. However, IR780 poor water solubility and acute cytotoxicity limit its direct use in cancer theragnostic. Herein, a novel Hyaluronic acid (HA)-based amphiphilic polymer was used, for the first time, in the preparation of polymeric nanoparticles (HPN) encapsulating IR780 aimed to be applied in breast cancer therapy. Furthermore, HPN co-encapsulating IR780 and Doxorubicin (DOX) were also produced in order to further enhance the therapeutic effectiveness of this nanoformulation. The results revealed that HPN were able to successfully encapsulate IR780 (IR-HPN) and the IR780-DOX combination (IR/DOX-HPN). Furthermore, the encapsulation of IR780 in HPN improved its absorption at 808 nm by about 2.2-fold, thereby enhancing its photothermal potential, as well as its cytocompatibility. The 2D in vitro cell uptake studies demonstrated that the nanostructures displayed a higher internalization by breast cancer cells than by normal cells. In addition, the assays performed in 3D in vitro models of breast cancer revealed that HPN can penetrate into spheroids. Furthermore, the 3D in vitro studies also demonstrated that the combined application of IR-HPN and NIR light was unable to induce cytotoxicity on spheroids. In contrast, IR/DOX-HPN produced a decrease on spheroids cells’ viability, and their combination with NIR light induced an even stronger therapeutic effect, thus revealing the potential of these nanoparticles for cancer chemo-phototherapy.
关键词： Cancer,Polymeric Nanoparticles,Chemotherapy,IR780,Photothermal therapy,Doxorubicin
更新于2025-11-21 11:08:12
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A Near Infrared-Modulated Thermosensitive Hydrogel for Stabilization of Indocyanine Green and Combinatorial Anticancer Phototherapy
摘要： Indocyanine green (ICG), a multifunctional near-infrared (NIR) imaging agent approved by the FDA, has been extensively used in clinical cancer theranosis, but limited by its inherent instability, short plasma half-life and lack of targeting ability. Herein, an in situ formed photothermal network based thermosensitive hydrogel (PNT-gel) constructed by supramolecular cross-linking conjugated polymers was developed for stabilization of ICG and efficient combinatorial photothermal/photodynamic antitumor therapy. While the conjugated polymeric backbone in PNT-gel anchored the aromatic phototherapeutic agent ICG via π–π stacking interactions to avoid premature leakage, it also directly converted low-dose NIR light to induce localized hyperthermia to enhance the photothermal effect. The PNT-gel shows a reversible gel-to-sol upper critical solution temperature (UCST) that is slightly above the body temperature. Therefore, the controlled release of ICG was switched on or off by NIR via photothermal-induced gel-sol transition. In vitro and in vivo antitumor experiments demonstrated that ICG loaded PNT-gel not only efficiently induced the killing of 4T1 cancer cells, but also achieved almost complete eradication of 4T1 cells by one-dose in combinatorial photothermal/photodynamic therapy under irradiation of a low-dose 808 nm laser (0.14 W cm-2). Additionally, the combinational therapy proved to enhance the effectiveness of photodestruction without tumor recurrence compared with intratumoral injection photothermal therapy (PTT) or photodynamic therapy (PDT) treatment alone.
关键词： Near Infrared-Modulated Thermosensitive Hydrogel,Combinatorial Anticancer Phototherapy,Indocyanine Green,Photodynamic Therapy,Photothermal Therapy
更新于2025-11-21 11:08:12
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Polydopamine-functionalized black phosphorus quantum dots for cancer theranostics
摘要： Black phosphorus (BP) is a promising theranostic agent owing to its excellent photothermal property, biocompatibility and biodegradability. However, the rapid degradation of BP with oxygen and moisture causes the innate instability that is the Achilles’ heel of BP, hindering its further applications in cancer theranostics. Herein, a facile surface passivation strategy was developed to prepare polydopamine (PDA) coated BP quantum dots (QDs) (denoted as BP@PDA) through self-polymerization method. PDA with enriched phenol groups plays as a scavenger of reactive oxygen, which can efficiently prevent the oxidation of BP quantum dots and make them much stable in water (～90% for BP@PDA vs. only 10% for pure BP QDs after 10 days storage). Furthermore, PDA with strong near-infrared (NIR) absorption could greatly improve the photothermal conversion efficiency (PCE) of BP QDs from 22.6% to 64.2% (～2.84-fold higher). Considering the excellent biodegradability and good biocompability of both BP QDs and PDA, the as-prepared BP@PDA hold great potential for cancer theranostics.
关键词： Photoacoustic imaging,Black phosphorus,Photothermal therapy,Cancer theranostics,Polydopamine
更新于2025-11-19 16:56:42
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Dopamine-Mediated Assembly of Citrate-Capped Plasmonic Nanoparticles into Stable Core-Shell Nanoworms for Intracellular Applications
摘要： Plasmonic nanochains, derived from the one-dimensional assembly of individual plasmonic nanoparticles (NPs), remain infrequently explored in biological investigations due to their limited colloidal stability, ineffective cellular uptake, and susceptibility to intracellular disassembly. We report the synthesis of polydopamine (PDA)-coated plasmonic “nanoworms” (NWs) by sonicating citrate-capped gold (Cit-Au) NPs in a concentrated dopamine (DA) solution under alkaline conditions. DA mediates the assembly of Cit-Au NPs into Au NWs within 1 min, and subsequent self-polymerization of DA for 60 min enables the growth of an outer conformal PDA shell that imparts stability to the inner Au NW structure in solution, yielding “core–shell” Au@PDA NWs with predominantly 4–5 Au cores per worm. Our method supports the preparation of monometallic Au@PDA NWs with different core sizes and bimetallic PDA-coated NWs with Au and silver cores. The protonated primary amine and catechol groups of DA, with their ability to interact with Cit anions via hydrogen bonding and electrostatic attraction, are critical to assembly. When compared to unassembled PDA-coated Au NPs, our Au@PDA NWs scatter visible light and absorb near-infrared light more intensely, and enter HeLa cancer cells more abundantly. Au@PDA NWs cross the cell membrane as intact entities primarily via macropinocytosis, mostly retain their inner NW structure and outer PDA shell inside the cell for 24 h, and do not induce noticeable cytotoxicity. We showcase three intracellular applications of Au@PDA NWs, including label-free dark-field scattering cell imaging, delivery of water-insoluble cargos without pronounced localization in acidic compartments, and photothermal killing of cancer cells.
关键词： 1D assembly,citrate-capped nanoparticles,polydopamine coating,photothermal killing,intracellular delivery,plasmonic nanoworms,dark-field scattering imaging
更新于2025-11-19 16:56:42
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Melanin-like nanoparticles decorated with an autophagy-inducing peptide for efficient targeted photothermal therapy
摘要： Photothermal therapy efficiently ablates tumors via hyperthermia but inevitably induces serious side effects including thermal damage to normal tissues, inflammations and enhanced risk of tumor metastasis. In this study, we fabricated a dual peptide decorated melanin-like nanoparticle for tumor-targeted and autophagy-promoted photothermal therapy in pursuit of improved cancer treatment. The multifunctional nanoparticle was composed of dual peptide RGD- and beclin 1-modified and PEGylated melanin-like polydopamine nanoparticles. Beclin 1-derived peptide modified on the nanoparticle up-regulated autophagy in cancer cells and further sensitized the photothermal ablation to tumors. RGD decorated on the particle surface enhanced the selectivity and cellular uptake of polydopamine nanoparticles by breast cancer cells. In vivo therapeutic experiments revealed that the tumor-targeted and autophagy promotion-associated photothermal therapy efficiently regressed tumors at a low temperature around 43 oC. The study provides a novel and efficient strategy to improve the efficiency of photothermal therapy via the up-regulation of autophagy in tumor cells.
关键词： photothermal therapy,beclin 1,multifunctional nanoparticles,autophagy,melanin-like
更新于2025-11-19 16:56:35