- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Short-Term Ultraviolet (UV)-A Light-Emitting Diode (LED) Radiation Improves Biomass and Bioactive Compounds of Kale
摘要： The aim of this study was to determine the influence of two types of UV-A LEDs on the growth and accumulation of phytochemicals in kale (Brassica oleracea var. acephala). Fourteen-day-old kale seedlings were transferred to a growth chamber and cultivated for 3 weeks. The kale plants were subsequently subjected to two types of UV-A LEDs (370 and 385 nm) of 30 W/m2 for 5 days. Growth characteristics were all significantly increased in plants exposed to UV-A LEDs, especially at the 385 nm level, for which dry weight of shoots and roots were significantly increased by 2.22 and 2.5 times, respectively, at 5 days of treatment. Maximum quantum efficiency of photosystem II photochemistry (Fv/Fm ratio) began to decrease after 3 h of treatment compared to the control. The total phenolic content of plants exposed to the two types of UV-A LEDs increased by 25% at 370 nm and 42% at 385 nm at 5 days of treatment, and antioxidant capacity also increased. The two types of UV-A LEDs also induced increasing contents of caffeic acid, ferulic acid, and kaempferol. The reactive oxygen species (ROS) temporarily increased in plants exposed to the two types of UV-A LEDs after 3 h of treatment. Moreover, transcript levels of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) genes and PAL enzyme activity were higher in plants treated with UV-A LEDs. Our results suggested that short-term UV-A LEDs were effective in increasing growth and improving antioxidant phenolic compounds in kale, thereby representing a potentially effective strategy for enhancing the production of phytochemicals.
关键词： transcript level,reactive oxygen species,phenolic compound,UV-A LEDs,kale,antioxidant capacity
更新于2025-09-11 14:15:04
-
The Radioprotective Effects of Low-Intensity Laser Radiation on Rat Peripheral Blood Cells
摘要： The radioprotective effect of low-intensity laser radiation on blood cells was studied on Wistar rats. The following procedures was carried out: single total body γ-radiation (dose of 3 Gy) of rats; over-vein irradiation with continuous laser radiation (λ = 670 nm); and combined γ- and laser irradiation, which varied among different series of experiments according to the number of procedures and the energy density of the laser radiation (2.5 J/cm2, 1.25 J/cm2). The influence of γ- and laser radiation on the number of peripheral blood cells in rats, the activity of antioxidant defense enzymes (superoxide dismutase and catalase), and on the blood absorption spectra in regions sensitive to its oxygenation were studied. On the 4th day after γ-irradiation, the radioprotective effect of low-intensity laser radiation was manifested as an increase in the average number of leukocytes (up to 1.4 times) and lymphocytes (up to 1.8 times) in comparison with reduced γ-irradiation, in an increase in the relative lymphocyte proportion in the leukocyte population (up to 1.5 times), and in an increase in the activities of catalase and superoxide dismutase, which were decreased by the γ-irradiation. It was found that the changes in the number of erythrocytes, leukocytes, lymphocytes, and superoxide dismutase activity initiated by γ-radiation alone and by combined laser and γ-radiation differed in individual rats and depended on individual initial values. The molecular mechanisms of the observed photo-radiobiological effects are discussed.
关键词： low-intensity laser radiation,blood absorption spectra,γ-radiation,blood cells,antioxidant defense enzymes
更新于2025-09-11 14:15:04
-
Synthesis, structure, luminescence and electrochemical and antioxidant properties of anion‐controlled silver(I) complexes with 2,2′‐(1,4‐butanediyl)bis‐1,3‐benzoxazole
摘要： To explore the influence of various anions on the self‐assembly and properties of silver complexes, reactions of anions of silver salts with 2,2′‐(1,4‐butanediyl)bis‐1,3‐benzoxazole (BBO) afforded four complexes, formulated as [Ag2(BBO)2(p‐toluenesulfonate)2] (1), {[Ag(BBO)(picrate)]}∞ (2), {[Ag(BBO)1/2(o‐coumarate)]·DMF}∞ (3) and {[Ag2(BBO)3](PF6)2}∞ (4). These complexes were characterized using elemental analysis, infrared spectroscopy and single‐crystal X‐ray diffraction. The crystal analysis results show that under the influence of coordination modes and steric hindrance of anions, the complexes exhibited binuclear (1), one‐dimensional polymeric (2 and 3) and two‐dimensional polymeric (4) structures. Compared with the BBO ligand, only complex 1 has a new emission peak at 428 nm, which is attributed to ligand–metal charge transfer. The emission peaks of complexes 2–4 are similar to those of the BBO ligand, which can be due to π–π* and n–π* transitions. These results indicate that anions can modulate the structures and luminescent properties of silver complexes. Moreover, cyclic voltammograms of 1–4 indicated an irreversible Ag+/Ag couple with the order of reversibility being 2 > 1 > 4 > 3. In vitro antioxidant experiments showed that complex 3 has significant antioxidant activity against superoxide and hydroxyl radicals.
关键词： luminescence,anion regulation,electrochemical property,antioxidant,Ag(I) complex
更新于2025-09-11 14:15:04
-
Different Postharvest Responses of Fresh-Cut Sweet Peppers Related to Quality and Antioxidant and Phenylalanine Ammonia Lyase Activities during Exposure to Light-Emitting Diode Treatments
摘要： The in?uence of emitting diode (LED) treatments for 8 h per day on functional quality of three types of fresh-cut sweet peppers (yellow, red, and green) were investigated after 3, 7, 11, and 14 days postharvest storage on the market shelf at 7°C. Red LED light (660 nm, 150 μmol m?2 s?1) reduced weight loss to commercially acceptable level levels (≤2.0%) in fresh-cuts of yellow and green sweet peppers at 7 and 11 d, respectively. Blue LED light (450 nm, 100 μmol m?2 s?1) maintained weight loss acceptable for marketing in red fresh-cut sweet peppers up to 11 d. Highest marketability with minimum changes in color di?erence (?E) and functional compounds (total phenols, ascorbic acid content, and antioxidant activity) were obtained in yellow and green sweet pepper fresh-cuts exposed to red LED light up to 7 and 11 d, respectively, and for red sweet pepper fresh-cuts exposed to blue LED light for 11 d. Red LED light maintained the highest concentrations of β carotene, chlorophyll, and lycopene in yellow, green, and red sweet pepper fresh-cuts up to 7 d. Similarly, blue LED light showed the highest increase in lycopene concentrations for red sweet pepper fresh-cuts up to 7 d. Red LED (yellow and green sweet peppers) and blue LED (red sweet pepper) lights maintained phenolic compounds by increasing phenylalanine ammonia lyase activity. Thus, the results indicate a new approach to improve functional compounds of di?erent types of fresh-cut sweet pepper.
关键词： postharvest quality,antioxidant activity,bioactive compounds,photo technology,Capsicum annuum L.,shelf life
更新于2025-09-11 14:15:04
-
Young’s double-slit interference enabled by surface plasmon polaritons: a review
摘要： Red yeast rice has been utilized for colouring food, brewing wine and preserving meat, which is also used as a folk medicine for centuries. In this study, a water-soluble non-starch polysaccharide from red yeast rice (RYRP) was extracted by using ultrasonic-assisted extraction method. By using Box–Behnken design (BBD) to optimize the parameters for extracting the RYRP, the maximum extraction yield (3.37%±0.78%) was obtained under the optimal extraction conditions as follows: ratio of water to raw material (40 mL/g), extraction temperature (62 °C), extraction time (75 min) and ultrasonic power (200 W). Moreover, monosaccharide composition analysis showed that RYRP was consisted of mannose, glucosamine, glucose and galactose with a molar ratio of 0.152:0.015:1:0.149. The molecular weight distribution analysis showed that the average molecular weight of the RYRP fraction was about 3.49 × 103 Da. Furthermore, RYRP exhibited significant antioxidant activities in vitro and the gastrointestinal-protective effect in vivo using gastrointestinal disorders (GD) model mice. RYRP could be explored as a potential source in the pharmaceutical and functional food industries.
关键词： Polysaccharide,Ultrasonic-assisted extraction,Red yeast rice,Antioxidant activities,Structural characterization,Gastrointestinal-protective effect
更新于2025-09-11 14:15:04
-
Inverted MAPbI <sub/>3</sub> Perovskite Solar Cells with Graphdiyne Derivative‐Incorporated Electron Transport Layers Exceeding 20% Efficiency
摘要： BACKGROUND: Slight acidic electrolyzed water (SAEW) has been widely used in cleaning systems of fruit and vegetables. It strongly reduces microbial contamination. However, no information is available on whether SAEW offers the potential for fresh jujube cleaning. The purposes of this study were first, to compare the effectiveness of SAEW with commercial sanitizers (i.e. NaClO or Ca(ClO)2) on ‘Jiancui’ jujube; second, to determine the response of fruit decay, tissue alcium content, and quality attributes to dip application of Ca(NO3)2 by concentration; and finally to investigate the effects of SAEW combined with calcium nitrate (Ca(NO3)2) or calcium acetate (Ca(OAc)2)) on fruit calcium uptake, quality attributes, and bioactive compounds. RESULTS: Fruits washed with NaClO, Ca(ClO)2 or an SAEW solution showed no difference in reduction of decay incidence. In contrast to NaClO treatment, SAEW or Ca(ClO)2 significantly retarded losses in fruit firmness (FF), green color (hue angle) and skin lightness (L*), and maintained intact pericarp tissue structure during storage at 1 °C. Application of Ca(NO3)2 at 5-10 g L-1 effectively promoted Ca2+ uptake and minimized declines in FF and L* value, but had no effect on decay development. Adding 10 g L-1 Ca(NO3)2 or Ca(OAc)2 to SAEW provided an additional benefit in increasing decay resistance, increasing Ca2+ into fruit, and increasing levels of bioactive compounds in jujube fruit. CONCLUSION: SAEW in combination with Ca(NO3)2 or Ca(OAc)2 has commercial potential for fresh jujube disinfection and improving storage quality as a result of the cleaning processes.
关键词： antioxidant capacity,calcium,Zizyphus jujuba Mill.,slight acidic electrolyzed water,storage quality,decay
更新于2025-09-11 14:15:04
-
UV-light intervened synthesis of imidazo fused quinazoline and its solvatochromism, antioxidant, antifungal and luminescence properties
摘要： The photochemical preparation of benzo[4,5]imidazo[2,1-b]quinazoline was assessed under the illumination of UV wavelength (>360 nm). The compound 3,4-dihydronaphthalen-1(2H)-one 1 with 1H-benzo[d]imidazol-2-amine 2 in the existence of KOH/DMF which lead to the aromatized compound 3. The notable remark of this reaction is that the UV irradiation (312 nm) plays a vital part in the formation of the compound, 3 in shorter duration (2h) which resulted in the reaction gave was higher yield (96%). Compound 3 was analyzed by H1, C13 NMR, and HRMS. Solvatochromism impacts of the compound 3 was computed with deference (DCM < methanol< DMF < DMSO). The compound, 3 was presented to invitro free radical screening utilizing DPPH strategy and furthermore H2O2 technique. The standard ascorbic acid has less IC50 value esteem than compound 3. Further, it was subjected to in vitro fungicidal action against two Aspergillus species (A. flavus & niger). The anti-fungal activity of compound 3 additionally demonstrated great action, when compared antifungal specialist Fluconazole.
关键词： solvatochromism,DPPH,UV radiation,anti-fungal,H2O2,antioxidant,Imidazo[2,1-b]quinazoline
更新于2025-09-10 09:29:36
-
Nanostructured TiO2-induced photocatalytic stress enhances the antioxidant capacity and phenolic content in the leaves of Vitis vinifera on a genotype-dependent manner
摘要： Over the past decades, nanotechnology has received great attention and brought revolutionary solutions for a number of challenges in scientific fields. Industrial, agricultural and medical applications of engineered nanomaterials have increased intensively. The ability of titanium dioxide nanoparticles (TiO2 NPs) to produce reactive oxygen species (ROS), when excited by ultra-violet (UV) light, makes them useful for effectively inactivate various pathogens. It is known that ROS also have signalling role in living organisms, therefore, TiO2 NPs-induced ROS can influence both enzymatic and non-enzymatic defence systems, and could play a role in the resistance of plants to pathogens. Herein, we studied the photocatalytic stress responses of grapevine (Vitis vinifera L.) as model plant, when exposed to a well-known photocatalyst, Degussa P25 TiO2 NPs. The photocatalytically produced ROS such as superoxide anion, hydroxyl radical and singlet oxygen were confirmed by electron paramagnetic resonance spectroscopy. Foliar exposure of five red cultivars (Cabernet sauvignon, Cabernet franc, Merlot, Kékfrankos and Kadarka) was carried out in blooming phenophase under field condition where plants are exposed to natural sunlight with relatively high UV radiation (with a maximum of ~ 45 W m-2). After two weeks of exposure, the effects of photogenerated ROS on the total phenolic content, antioxidant capacity, flavonol profile and the main macro-, microelements of the leaves were studied in detail. We found that foliar application of TiO2 NPs boosted the total phenolic content and biosynthesis of the leaf flavonols depending on the grapevine variety. Photocatalytically active TiO2 NPs also increased K, Mg, Ca, B and Mn levels in the leaves as shown by ICP-AES measurements.
关键词： micro- and macroelements,photocatalytic stress,antioxidant capacity,grape leaf polyphenols,flavonols
更新于2025-09-10 09:29:36
-
Changes in the photosynthesis properties and photoprotection capacity in rice (Oryza sativa) grown under red, blue, or white light
摘要： Non-photochemical quenching (NPQ) of the excited state of chlorophyll a is a major photoprotective mechanism plants utilize to survive under high light. Here, we report the impact of long-term light quality treatment on photosynthetic properties, especially NPQ in rice. We used three LED-based light regimes, i.e., red (648–672 nm), blue (438–460 nm), and “warm” white light (529–624 nm), with the incident photon flux density of 300 μmol photons m?2 s?1, the difference in the absorbed photon flux densities by leaves grown under different light quality being less than 7%. Our results show that blue light, as compared to white light, induced a significant decrease in Fv/Fm, a decreased rate of reduction of P700+ after P700 was completely oxidized; furthermore, blue light also induced higher NPQ with an increased initial speed of NPQ induction, which corresponds to the qE component of NPQ, and a lower maximum quantum yield of PSII, i.e., Y(II). In contrast, rice grown under long-term red light showed decreased Y(II) and increased NPQ, but with no change in Fv/Fm. Furthermore, we found that rice grown under either blue or red light showed decreased transcript abundance of both catalase and ascorbate peroxidase, together with an increased H2O2 content, as compared to rice grown under white light. All these data suggest that even under a moderate incident light level, rice grown under blue or red light led to compromised antioxidant system, which contributed to decreased quantum yield of photosystem II and increased NPQ.
关键词： Non-photochemical quenching of the excited state of chlorophyll a,Effective quantum yield of PSII,Light quality,Quantum yield of regulated energy dissipation in PSII,Oryza sativa,Antioxidant system
更新于2025-09-10 09:29:36
-
Preparation of α-Linolenic-Acid-Loaded Water-in-Oil-in-Water Microemulsion and Its Potential as a Fluorescent Delivery Carrier with a Free Label
摘要： Our previous work has demonstrated that α-linolenic acid (ALA)-loaded oil-in-water (O/W) microemulsion could enhance ALA antioxidant capacity. Meanwhile, we also observed that synthesized microemulsion itself had ?uorescence. In this work, we have prepared a multiple water-in-oil-in-water (W/O/W) microemulsion to further enhance ALA antioxidant capacity and activate this delivery carrier application potential with a free label. The compositions of primary water-in-oil (W/O) microemulsion were obtained using pseudo-ternary phase diagrams, and then W/O/W microemulsion was prepared adopting the “two-step heterotherm method”. The conductivity of W/O/W microemulsion was measured to lie between 250.0 and 350.0 μs/cm. The spherical droplets with a mean particle diameter of 10.0?20.0 nm were con?rmed by transmission electron microscopy and dynamic light scattering. Nuclear magnetic resonance con?rmed that ALA di?used to the multiple water?oily interface simultaneously. In addition, the in vitro release and antioxidant capacity measurements of ALA-loaded W/O/W microemulsion concluded the sustained-release e?ect and excellent antioxidant capacity. The ?uorescent intensity of W/O/W microemulsion was markedly increased in comparison to O/W microemulsion. The synthesized microemulsion could lead to important applications and have advantages of a label-free ?uorescent carrier for optical imaging purposes.
关键词： ALA-loaded W/O/W microemulsion,?uorescent delivery carrier,characterization,antioxidant capacity
更新于2025-09-10 09:29:36