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Liquid Crystal-Induced Myoblast Alignment
摘要： The ability to control cell alignment represents a fundamental requirement toward the production of tissue in vitro but also to create biohybrid materials presenting the functional properties of human organs. However, cell cultures on standard commercial supports do not provide a selective control on the cell organization morphology, and different techniques, such as the use of patterned or stimulated substrates, are developed to induce cellular alignment. In this work, a new approach toward in vitro muscular tissue morphogenesis is presented exploiting liquid crystalline networks. By using smooth polymeric films with planar homogeneous alignment, a certain degree of cellular order is observed in myoblast cultures with direction of higher cell alignment corresponding to the nematic director. The molecular organization inside the polymer determines such effects since no cell organization is observed using homeotropic or isotropic samples. These findings represent the first example of cellular alignment induced by the interaction with a nematic polymeric scaffold, setting the stage for new applications of liquid crystal polymers as active matter to control tissue growth.
关键词： liquid crystalline alignments,liquid crystalline network,cell alignment,biomaterials,muscular tissue engineering
更新于2025-11-21 11:01:37
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Silk Fibroin-Based Films Enhance Rhodamine 6G Emission in the Solid State: A Chemical-Physical Analysis of their Interactions for the Design of Highly Emissive Biomaterials
摘要： Described herein is the preparation of dye-doped films employing silk fibroin (SF) as a biomaterial, capable of preserving the optical properties of the monomeric dye in the solid state, a critical requisite for optical and biolaser applications. A comprehensive physical–chemical characterization is reported for SF films doped with Rhodamine 6G, an ideal candidate for photonics and optoelectronics. Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD) and X-Ray diffraction (XRD) provide information on SF secondary conformation in the presence of rhodamine. UV–vis absorption spectra and exciton CD inform on the structure of encapsulated rhodamine, while changes in dye photophysical properties illuminate the molecular mechanism of the involved host–guest interactions. SF host environment inhibits rhodamine dimer formation, indicating that SF is an optimum matrix to keep rhodamine essentially monomeric at concentrations as high as 7 mm in the film. The relevant optical properties of these films and the easiness of their preparation, make these systems optimal candidates for innovative photonic technologies.
关键词： FTIR,silk fibroin,dye-doped biomaterials,exciton coupled CD,fluorescence
更新于2025-09-23 15:23:52
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Long-Term Stability of Two Thermoplastic Polymers Modified with Silver Nanoparticles
摘要： The aim of this study was to investigate the mechanical properties of polymeric composites prepared via extrusion and injection moulding. Four stable thermoplastic polymers were used as composites matrices (two kinds of polymethyl methacrylate and two kinds of co-polymer acrylonitrile-butadiene-styrene). Silver nanoparticles AgNPs were used as a modifying phase. Mechanical properties of testes materials were determined during the uniaxial stretching. Surface properties such as roughness and contact angle were also evaluated. The materials’ stability was assessed using scanning electron microscopy and non-destructive ultrasonic testing. All measurements were carried out at time intervals, determining both the initial parameters and after 6 and 12 months of incubation in deionized water. The obtained results proved that neither the preparation technology nor the amount of the modi?er adversely affect the mechanical properties of the tested composites. The incorporated modi?er does not change the surface properties signi?cantly. The studies conducted after the materials’ incubation in water indicate their stability.
关键词： biomaterials,silver nanoparticles,biostable polymers
更新于2025-09-23 15:22:29
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The synthesis of CdSe quantum dots stabilized by polymers and polyelectrolyte capsules
摘要： The formation of nanocomposites based on quantum dots and polymers is a way of combining the benefits of quantum dots, such as their fundamental novelty and biomedical perspectives, with technological capabilities of polymers. The aim of this work was to synthesize cadmium selenide (CdSe) quantum dots in the presence of polymers (polyethyleneimine and chitosan) in an aqueous solution and embed cadmium selenide/polymer nanoparticles into polyelectrolyte capsules. To characterize the polymer-stabilized cadmium selenide quantum dots, the authors used fluorescence spectroscopy and dynamic and electrophoretic light scattering methods. The morphology of cadmium selenide/polymer nanoparticles characterized by the method of transmission electronic microscopy. The incorporation of quantum dots into a polymer matrix can significantly reduce their toxicity and prolong the duration of their fluorescence signals. Cadmium selenide nanoparticles stabilized by chitosan were used to synthesize five-layered polyelectrolyte capsules.
关键词： nanoparticles,polymers,biomaterials
更新于2025-09-23 15:21:01
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Optoregulated biointerfaces to trigger cellular responses
摘要： Optoregulated biointerfaces offer the possibility to manipulate the interactions between cell membrane receptors and the extracellular space. This Feature Article summarizes recent efforts of our group and others during the last decade in the development of light-responsive biointerfaces to stimulate cells and elicit cellular responses using photocleavable protecting groups (PPG) as working tool. This article begins by providing a brief introduction to available PPGs, with a special focus on the widely used o-nitrobenzyl family, followed by an overview of molecular design principles for the control of bioactivity in the context of cell-materials interactions, and the characterization methods to follow the photoreaction at surfaces. We present various light guided cellular processes using PPGs, including cell adhesion, release, migration, proliferation and differentiation, in vitro and in vivo. Finally, this feature article closes with our perspective view on the current status and future challenges of this topic.
关键词： cell-materials interactions,photoresponsive biomaterials,phototriggers,light-guided cells
更新于2025-09-23 15:21:01
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Hybrid Ceramo-Polymeric Nano-Diamond Composites
摘要： This paper discusses a new class of bio-mechanical scaffolds active for tissue engineering based on a nano-diamond-filled hydrophilic polymer matrix. The new biomaterials used have special mechanical and biological properties for which they should be extensively studied for their use for various advanced biomedical applications. The new hybrid material has been prepared using 2 and 5% by volume of detonating nano-diamonds and poly (hydroxy-ethyl-methacrylate) hydrophilic. Both the mechanical and biological properties specific to the nanocomposite are hybrids in nature. The paper presents the analytical procedures of the hybrid material and the preliminary mechanical characterization. This class of hybrid materials has a high potential for biomimetic, osteoconductive and osteoinductive applications as active bio-mechanical bones for increasing osteoblasts and differentiating stem cells. At the same time, these hybrid nano-composites possess a much improved mechanical strength that exceeds the mechanical deficiencies of the hydrogels traditionally used for bone regeneration and can be applied as an osteoinductive coating for metal trabecular scaffolds. Micro-trabecular metal structures coated with active and osteoinductive biomechanical ceramic-polymeric biomechanical scaffolds are proposed to recreate macro and micro-distribution of bone stresses and deformations.
关键词： Biomaterials,Biomimetic,Nano-Composites
更新于2025-09-23 15:21:01
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Immunofunctional Photodegradable Poly(ethylene glycol) Hydrogel Surfaces for the Capture and Release of Rare Cells
摘要： Circulating tumor cells (CTCs) play a central role in cancer metastasis and represent a rich source of data for cancer prognostics and therapeutic guidance. Reliable CTC recovery from whole blood therefore promises a less invasive and more sensitive approach to cancer diagnosis and progression tracking. CTCs, however, are exceedingly rare in whole blood, making their quantitative recovery challenging. Several techniques capable of isolating these rare cells have been introduced and validated, yet most suffer from low CTC purity or viability, both of which are essential to develop a clinically viable CTC isolation platform. To address these limitations, we introduce a patterned, immunofunctional, photodegradable poly(ethylene glycol) (PEG) hydrogel capture surface for the isolation and selective release of rare cell populations. Flat and herringbone capture surfaces were successfully patterned via PDMS micromolding and photopolymerization of photolabile PEG hydrogels. Patterned herringbone surfaces, designed to convectively transport cells to the capture surface, exhibited improved capture density relative to flat surfaces for target cell capture from buffer, buffy coat, and whole blood. Uniquely, captured cells were released for collection by degrading the hydrogel capture surface with either bulk or targeted irradiation with cytocompatible doses of long wavelength UV light. Recovered cells remained viable following capture and release and exhibited similar growth rates as untreated control cells. The implementation of molded photodegradable PEG hydrogels as a CTC capture surface provides a micropatternable, cytocompatible platform that imparts the unique ability to recover pure, viable CTC samples by selectively releasing target cells.
关键词： circulating tumor cell,biomaterials,immunocapture,microfluidics
更新于2025-09-23 15:21:01
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Controlled aggregation of Ag nanoparticles on oxide templates on nitinol by electrodeposition
摘要： Nitinol is an unusual material for hard-tissue replacements because of their unique superelasticity, biocompatibility, and low elastic modulus. Nitinol surface was modified by plasma electrolytic oxidation with an aim to produce oxide film on the surfaces for inhibiting Ni ion release. In addition, various Ag structures were constructed on the oxide templates on nitinol using electrodeposition in an AgC2H3O2-based solution for improving the antibacterial ability. Control of the electrolyte by collagen concentration caused a change in Ag shape and aggregation. Preferred growth along Ag (111) by orientated attachment of the Ag led to the formation of Ag dendrites in the AgC2H3O2 solution. Spherical Ag nanostructures, which were well dispersed on the templates, were obtained by electrodeposition in an AgC2H3O2-collagen solution on templates. Increasing the concentration of collagen caused aggregated Ag to form coarse spherical structures with a non-uniform distribution.
关键词： Deposition,shape memory alloys (SMA),Biomaterials,Electrochemistry
更新于2025-09-23 15:19:57
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Antimicrobial biomaterials and their potential application in ophthalmology
摘要： Infections associated with the use of intraocular, periocular, or orbital implants are associated with an increase in both morbidity and in the costs of ophthalmological surgery. This is due to an increased number of visits and the need for additional treatments, at a time when some conventional therapies are losing their efficacy, or even hospitalization. To avoid such consequences, the first step should be to prevent the biomaterials that form implants from being colonized by various microorganisms, either intraoperatively or postoperatively. To this end, several lines of research have emerged that aim at equipping implants with antimicrobial properties, some of which are described in this review.
关键词： Ophthalmology,Implant-associated infections,Antimicrobial biomaterials
更新于2025-09-19 17:15:36
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A highly sensitive living probe derived from nanoparticle-remodeled neutrophils for precision tumor imaging diagnosis
摘要： Timely and precise diagnosis of malignant tumors is of great value to patients’ treatment and prognosis. Although nanotechnology-based molecular imaging represents a major advancement in the field of tumor imaging diagnosis, it is restricted by the rapid blood clearance of nanoparticles and the diverse physiological barriers in vivo; hence, its further application is greatly hindered. Cell carriers, ascribed to their natural biological properties, are thus gaining increasing attention for addressing such issues. Here, taking full advantage of the inflammation-homing capability of neutrophils, we constructed a highly sensitive cell probe in which reduced bovine-serum albumin (BSA) nanoparticles, loaded with imaging agents (gadolinium (Gd) and BODIPY), were covalently fixed onto the cellular surface by 5-thio-2-nitrobenzoate (TNB)-mediated fast and efficient disulfide–thiol exchange. Impressively, the remodeling process exerted a negligible effect on the neutrophils’ biological profiles with regard to cell viability, morphology, and cell-surface protein markers. Compared with nanoparticle-based imaging agents in a lung-cancer xenograft model, the living neutrophil probe demonstrated faster targeting and stronger accumulation in the tumor site, as revealed by fluorescence and magnetic-resonance (MR) imaging. These results indicate the great potential of neutrophil-based living probe for precision tumor-diagnosis applications.
关键词： Biomaterials,Neutrophils,Nanoparticles,Molecular imaging,Tumor imaging
更新于2025-09-16 10:30:52